Journal: Cellular and Molecular Immunology

Article Title: Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2

doi: 10.1038/s41423-022-00924-8

Figure Lengend Snippet: Substitution of S982A and L18F weakens the generation of binding antibodies. The area under the curve (AUC) of anti-spike ( A ) and anti-RBD ( B ) IgG induced by the 19 designed groups of DNA immunogens ( n = 3 for each group). The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8. Data were analyzed for statistical significance using the unpaired Student’s t test. Data are shown as the mean with SD in each group. Dotted black lines indicate the limit of quantification (LOQ). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: EBioMedicine

Article Title: Waning immune responses against SARS-CoV-2 variants of concern among vaccinees in Hong Kong

doi: 10.1016/j.ebiom.2022.103904

Figure Lengend Snippet: Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group.

Techniques: Enzyme-linked Immunosorbent Assay, Inhibition, Neutralization, Ex Vivo, Expressing, MANN-WHITNEY

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Prognostic Value of Serum Epstein-Barr Virus Antibodies and Their Correlation with TNM Classification in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma

doi: 10.4143/crt.2020.1298

Figure Lengend Snippet: Baseline characteristics of patients according to EBV antibody levels

Article Snippet: The EBNA1-IgA and Zta-IgA levels were assessed according to the ELISA OD values according to the manufacturers’ instructions (Shanghai Jining Shiye Co., Ltd., Shanghai, China; Zhongshan Biotechnology Co., Ltd., Zhongshan, China).

Techniques:

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Prognostic Value of Serum Epstein-Barr Virus Antibodies and Their Correlation with TNM Classification in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma

doi: 10.4143/crt.2020.1298

Figure Lengend Snippet: Correlations between Epstein-Barr virus biomarker levels and the AJCC staging system, eighth edition

Article Snippet: The EBNA1-IgA and Zta-IgA levels were assessed according to the ELISA OD values according to the manufacturers’ instructions (Shanghai Jining Shiye Co., Ltd., Shanghai, China; Zhongshan Biotechnology Co., Ltd., Zhongshan, China).

Techniques: Virus, Biomarker Discovery

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Prognostic Value of Serum Epstein-Barr Virus Antibodies and Their Correlation with TNM Classification in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma

doi: 10.4143/crt.2020.1298

Figure Lengend Snippet: Univariable analyses of prognostic factors for the whole cohort of patients with nasopharyngeal carcinoma

Article Snippet: The EBNA1-IgA and Zta-IgA levels were assessed according to the ELISA OD values according to the manufacturers’ instructions (Shanghai Jining Shiye Co., Ltd., Shanghai, China; Zhongshan Biotechnology Co., Ltd., Zhongshan, China).

Techniques:

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Prognostic Value of Serum Epstein-Barr Virus Antibodies and Their Correlation with TNM Classification in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma

doi: 10.4143/crt.2020.1298

Figure Lengend Snippet: Multivariate analysis of prognostic factors for the whole cohort of patients with nasopharyngeal carcinoma

Article Snippet: The EBNA1-IgA and Zta-IgA levels were assessed according to the ELISA OD values according to the manufacturers’ instructions (Shanghai Jining Shiye Co., Ltd., Shanghai, China; Zhongshan Biotechnology Co., Ltd., Zhongshan, China).

Techniques:

Journal: bioRxiv

Article Title: Portable low-cost optical density meter

doi: 10.1101/2021.05.14.444207

Figure Lengend Snippet: Blue food dye dilution series for three samples (D1, D2, and D3), comparing OD values obtained from the OD meter to values from a SpectraMax M3 spectrophotometer at 590 nm. At each step in the dilution series, the dye concentration was reduced by 20%, down to OD values of 0.08, 0.07, and 0.05 for D1, D2, D3, respectively. The dark references for samples D1 and D2 were acquired with cuvettes filled with blue dye ( I dark = 70 and 73, respectively). The dark reference for sample D3 was acquired using the 3D printed light blocking insert ( I dark = 22). The blank reference for all samples was acquired with pure water in a cuvette ( I blank = 1000, 1019, 980, for D1, D2, D3, respectively). Dashed lines are linear fits to the data points of the same color up to the OD range of each plot. (A) OD values up to 2.2. In this range the I dark calibration for D3 leads to underestimating the OD values above OD 1.1 (green points and line). The optimal I dark for this sample is 60, which properly linearizes the curve (purple points and line). (B) OD values up to 1.1. In this range the underestimation of OD in sample D3 due to the incorrect I dark calibration is not significant.

Article Snippet: At each step in the dilution series, the dye concentration was reduced by 20%, down to OD values of 0.08, 0.07, and 0.05 for D1, D2, D3, respectively. shows three dilution curves of blue food dye, with the SpectraMax OD value in the X axis and the low-cost device OD value in the Y axis.

Techniques: Spectrophotometry, Concentration Assay, Blocking Assay